Biotech Industrial Training

To Bridge the skill gap between Industry and Academia

Dr. B. Lal Institute of Biotechnology offers comprehensive & in-depth practical hands-on Biotech training, in a real-life environment, which enables the participants to master the skills in Biotechnology. the mission of Biotech Industrial Training Programme is to provide industry-specific training to students, research scholars and faculty members for skill development and enhancing their job opportunities in the biotech industry. The Indian Biotechnology sector is one of the fastest growing knowledge-based sectors and is expected to play a key role in shaping India’s rapidly developing economy. In biotechnology industrial sector, a huge importance is given to the skilled manpower employed and hence, head-hunting has always been one of the prime priorities. Unfortunately, most of the students of biotechnology lag behind and fail to satisfy the skill requirement of these industries even though they may be strong in theoretical concepts. Hence, it has been marked at various forums that the freshly graduating students are often not – employable though trainable.

The Biotech Industrial Training Program aims to provide industry specific training to students for skill development and is specially designed for students to gain ‘hands-on’ training experience hence enhancing their job opportunities in biotech industry. Hence, all possible efforts are made to inculcate research aptitude and good work culture among the students through well planned, properly executed and evaluated training.

Standard (15 days)


Advanced Genetic Engineering(AGE) (Standard)

Module Content
Preparation of Molecular Grade Solution
Isolation of genomic DNA from prokaryotic sample
Isolation of genomic DNA from eukaryotic sample
Isolation of plasmid DNA from prokaryotic sample
Agarose Gel Electrophoresis and Quantification of DNA sample
Determination of melting temperature (Tm) of DNA
PCR Amplification using RAPD markers and Agarose gel electrophoresis
Restriction Digestion of DNA/RFLP

Biochemistry (BS)

Module Content
Introductory Lecture - Demonstration of Instruments, Laboratory mathematics, Good Laboratory Practices and Safe Handling of Biomedical Waste
Solution Preparation
Qualitative determination of carbohydrates, proteins and lipids
Quantitative estimation of lipids by Sulpho-Phospho-Vanillin method
Determination of sugar content by Dubois et al method
Quantitative estimation of total protein by Lowry method
Quantitative estimation of total protein by Lowry method
Separation of serum and plasma from blood
Isolation of Serum Protein by Salting out Method
Protein profiling by SDS- PAGE
Mid evaluation (Quiz or viva)
Report preparation, Viva and Presentation

Bioinformatics (BS)

Module Content
General Introduction to bioinformatics and Databases
To retrieve FASTA sequence from Various database
To perform BLAST of a protein sequence
Phylogenetic study
To perform molecular modelling using SwissModel
To validate the model structure of HIV-1 protease using Swiss model and PROCHECK
Model validation using various parameters
To find the binding pockets of the protein using CASTp
To perform change the format of molecule from mol to PDB
To perform molecular docking using AutoDock-4.2.5.1
To analyse the docked complex using AutoDock-4.2.5.1
Concluding lecture and discussion
Presentations, Viva-voice and report submission

Environmental Biotechnology (EB)

Module Content
Orientation Program, General Introduction to environmental biotechnology
Lab Visit: Proper Demonstration of Instruments and Good Laboratory Practices
Preparation of Buffers and Reagents and Introduction to lab mathematics
Determination Procedure of Physical parameter of water quality testing by HANNA EDGE
  • pH
  • Temperature
  • Electrical conductivity
  • Colour&odour
  • TDS
  • DO
  • Salinity
  • Determination Procedure of Chemical Parameter of water quality testing
  • Determination of acidity
  • Determination of alkalinity
  • Estimation of the hardness
  • Determination of the dissolved oxygen (Manual Method)
  • Determination Procedure of wastewater quality parameter
  • Determination of total, suspended, dissolved solids
  • Estimation of Nitrates Nitrogen
  • Determination of Ammonical Nitrogen
  • Determination of Biochemical oxygen demand
  • Determination of Chemical oxygen demand
  • Determination of Total phosphates
  • Determination of Sulphates
  • Enumeration of microbial index by standard plate count method( SPC)
  • Enumeration of Total coliforms by Multiple tube Fermentation test(MPN)
  • Report Writing, final monitoring by Quiz/ Viva/ Presentation

  • Food Microbiology (FM)

    Module Content
    Introduction to Food Microbiology
    General rules & regulations & instrumentation
    Isolation and Identification of common microorganism Spoiling Food

    (a)Morphology Characterization : Colony Morphology

    (b)Microscopic Characterization : Grams Staining

    (c)Biochemical Characterization by Bergey’s manual

    Detection of number of bacteria in milk by SPC ( Standard Plate count Method)
    Enumeration of Coliforms, Faecal Coliforms and E.coli in milk by using MPN (Multiple tube fermentation) Method
    Qualitative Production of Aflatoxin from Fungi
    Shelf life testing of routinely used edible product

    (a) Adulteration in milk

    Sampling of Probiotic bacteria from food samples and isolation on specialized enriched media
    Preparation of fermented food - Sauerkraut
    Lipase Production Test (Lipid and Fat Hydrolysis by Microbes)
  • Determination of Protein in food sample
  • Determination of Protein in food sample
  • Quality test of milk by MBRT test
    Report writing & viva

    Herbal Drug Formulations (HDF)

    Module Content
    Orientation Program, General Interaction and Introductory Lecture
    Lab Visit: Proper Utilization of Resources and Good Laboratory Practices
    Preparation of plant material for Herbal extract preparation
    Extraction techniques to prepare Herbal extract from Medicinal plants
    Qualitative phytochemical screening
    Total Phenolic Content Determination
    Total Flavonoid content Determination
    Total Antioxidant Activity
    Antimicrobial activity
    Presentation, Report Submission and Viva voce

    Immunotechnology (I)

    Module Content
    Introductory Lecture - Demonstration of Instruments, Laboratory mathematics, Good Laboratory Practices and Safe Handling of Biomedical Waste
    Determination of Blood group
    Immunodiffusion Techniques

    i. Radial Immunodiffusion Technique(RID)

    ii. Ouchterlony Double Diffusion Technique (ODD)

    Immunoelectrophoresis Techniques

    i. Rocket Immuno-electrophoresis Technique (RIEP)

    ii. Counter Current Immuno-electrophoresis Technique (CCIEP)

    Separation of serum and plasma Isolation of Serum Protein by Salting out Method.
    Serum Protein profiling by SDS- PAGE.
    Presentation, Report Submission and Viva voce

    Medical Microbiology (MM)

    Module Content
    Introductory Lecture
    Lab Visit: Proper Utilization of Resources and Good Laboratory Practices
    Introduction to Good Laboratory Practices and Instrumentation Handling of Specimens and Biomedical Waste Disposal Introduction to Normal Flora of Human Body
    Culture Media
  • Types of Culture Media
  • Preparation of Culture Media
  • Isolation of Pathogenic Bacteria from Clinical Samples
  • Serial Dilution Method
  • Spread Plate Method
  • Pour Plate Method
  • Streak Plate Method
  • Morphological Characterization of Microorganisms
  • Colony Characterization
  • Colony Forming Unit (CFU) Count
  • Microscopic Characterization of Microorganisms
  • Differential Staining
    • Gram’s Staining
    • Acid Fast Bacilli (AFB) Staining
    • Endospore Staining
  • Negative Staining
    • Capsule Staining
    Biochemical Characterization for Bacterial Identification
  • IMViC Test
  • Oxidase Test
  • Catalase Test
  • Isolation and Identification of Fungi
    Antibiotic Susceptibility Tests
  • Disc Diffusion Test
  • Enumeration of Bacteria: Growth Kinetic Study
    Presentation, Report Submission and Viva-Voce

    Molecular Biology (MB)

    Module Content
    Preparation of Molecular grade solutions
    Demonstration of isolation of genomic DNA from prokaryotes
    Demonstration of isolation of genomic DNA from eukaryotes
    Demonstration of plasmid DNA from prokaryotes
    Agarose gel electrophoresis
    Quantification of DNA sample
    Determination of melting temperature (Tm) of prokaryotic DNA
    Restriction Digestion/RFLP
    RAPD

    Nano Biotechnology (NB)

    Module Content
    PDA Media Preparation
    Microscopic identification of fungi by Lacto phenol cotton blue (LCB) staining method
    Inoculation of Fungus in PDA Media
    Preparation of cationic liposome bulb.
    Entrapment of protein in the liposome.
    Entrapment of DNA in the liposome.
    Estimating entrapment efficiency of Protein. Transfer the fungus in to autoclave distilled water
    Estimating entrapment efficiency of DNA.
    Rapid Synthesis of Silver Nanoparticles (from Fungi)
    Characterization of Silver Nanoparticles by U.V Visible Spectrophotometer

    Plant Biotechnology (PB)

    Module Content
    Orientation Program, General Interaction and Introductory Lecture
    Lab Visit: Proper Utilization of Resources and Good Laboratory Practices
    Preparation of PTC hood, glassware sterilization.
    Preparation of Hormone Stocks Solutions Preparation of Culture Media
    Explant Preparation and Surface Sterilization
    Callus Culture
    Embryo Culture
    Anther Culture
    Meristem Culture
    Micropropagation by Adventitious Bud Culture
    Micropropagation by Shoot Tip (Explant)
    Seed sterilization, inoculation and morphology study of seedling grown in vitro
    Isolation and observation of Plant Protoplast from leaves
    (Single cell culture) using Bergman’s cell plating technique and filter paper raft technique, micro chamber technique
    Preparation of protoplast and its fusion.
    Encapsulation of embryo for the preparation of artificial seeds
    Cell suspension culture
    Preparation, preservation and maintenance of Cell lines
    Presentation, Report Submission and Viva voce

    Specialized (30 days)


    Advanced Genetic Engineering(AGE) (Standard)


    Module Content
    Preparation of Molecular grade solutions
    Demonstration of isolation of genomic DNA from prokaryotes
    Demonstration of isolation of genomic DNA from Eukaryotes (animal and plant cells)
    Isolation of plasmid DNA from prokaryotes
    Agarose gel electrophoresis and quantification of molecular weight of DNA sample
    Tm calculation of DNA
    PCR amplification – gene specific
    PCR amplification – using RAPD Primers
    Multiplex PCR
    Colony PCR
    PCR Purification
    Competent cell preparation and transformation
    Ligation
    RFLP
    SNP detection
    SDS-Page

    Bioinformatics (Specialized)

    Module Content
    General Introduction to bioinformatics and Databases
    Demonstration of isolation of genomic DNA from prokaryotes
    To perform BLAST of a protein sequence
    Phylogenetic study
    To perform molecular modelling using SwissModel
    To validate the model structure of HIV-1 protease using Swiss model and PROCHECK
    Model validation using various parameters
    To find the binding pockets of the protein using CASTp
    To Draw the structure of carbamic acid using ChemSketch
    To perform change the format of molecule from molto PDB
    To perform molecular docking using AutoDock-4.2.5.1
    To analyse the docked complex using AutoDock-4.2.5.1
    To predict ADMET properties using chemicalize online tool
    Protein-protein interaction
    Primer designing
    Introduction to QSAR
    Demonstration to MD simulation using GROMACS
    Concluding lecture and discussion
    Presentations, Viva-voice and report submission

    Environmental Biotechnology (Specialized) (EB)

    Module Content
    Orientation Program, General Introduction to environmental biotechnology
    Lab Visit: Proper Demonstration of Instruments and Good Laboratory Practices
    Preparation of Buffers and Reagents and Introduction to lab mathematics
    Determination Procedure of Physical Parameter of water quality testing by HANNA EDGE
  • pH
  • temperature
  • Electrical conductivity
  • Colour&odour
  • TDS
  • DO
  • Salinity
  • Determination Procedure of Chemical Parameter of water quality testing
  • Determination of acidity
  • Determination of alkalinity
  • Estimation of the hardness
  • Determination of the dissolved oxygen (Manual Method)
  • Determination Procedure of wastewater quality parameter
  • Determination of total, suspended, dissolved solids
  • Estimation of Nitrates Nitrogen
  • Determination of Ammonical Nitrogen
  • Determination of Total Kjeldahl Nitrogen
  • Determination of Biochemical oxygen demand
  • Determination of Chemical oxygen demand
  • Determination of Total phosphate
  • Determination of Sulphate
  • Enumeration of microbial index by standard plate count method( SPC)
  • Enumeration of Total coliforms by Multiple tube Fermentation test(MPN)
  • Exercise based on Bioremediation of Industrial effluent
  • Visit of Industrial tour – Saras Dairy
  • Visit of Vermifiltration Plant- BIBT Campus
  • Report Writing, final monitoring by Quiz/ Viva/ Presentation

    Medical Microbiology (MM)

    Module Content
    Introductory Lecture
    Introduction to Good Laboratory Practices and Instrumentation
    Handling of Specimens and Biomedical Waste Disposal
    Introduction to Normal Flora of Human Body
    Culture Media
  • Types of Culture Media
  • Preparation of Culture Media
  • Isolation of Pathogenic Bacteria from Clinical Samples
  • Serial Dilution Method
  • Spread Plate Method
  • Pour Plate Method
  • Streak Plate Method
  • Morphological Characterization of Microorganisms
  • Colony Characterization
  • Colony Forming Unit (CFU) Count
  • Microscopic Characterization of Microorganisms
  • Differential Staining
    • Gram’s Staining
    • Acid Fast Bacilli (AFB) Staining
    • Endospore Staining
  • Negative Staining
    • Capsule Staining
    Biochemical Characterization for Bacterial Identification
  • IMViC Test
  • Urease Test
  • Triple Sugar Iron (TSI) Test
  • Oxidase Test
  • Catalase Test
  • Coagulase Test
  • Isolation and Identification of Fungi
    Antibiotic Susceptibility Tests
  • Disc Diffusion Test
  • Broth Dilution Test
  • E-Test
  • Enumeration of Bacteria: Growth Kinetic Study
    Automation in Medical Microbiology (Demonstration Only)
  • VITEK
  • BACTEC MGIT 320 (TB Culture)
  • BACTEC MGIT 9050 (Blood Culture)
  • Alifex (Urine Analysis – For Early Detection)
  • Presentation, Report Submission and Viva-Voce

    Molecular Biology (Specialized) (MB)

    Module Content
    Preparation of Molecular grade solutions
    Demonstration of isolation of genomic DNA from prokaryotes
    Demonstration of isolation of genomic DNA from Eukaryotes (animal and plant cells)
    Isolation of plasmid DNA from prokaryotes
    Agarose gel electrophoresis and quantification of DNA sample
    Calculation of DNA melting temperature and estimation of composition of nucleotide sample
    PCR amplification – gene specific
    PCR amplification – using RAPD Primers
    Multiplex PCR
    Colony PCR
    PCR Purification
    Competent cell preparation and transformation
    Ligation
    RFLP
    SNP detection
    SDS-Page

    Molecular Diagnostics (Specialized) (MD)

    Module Content
    Orientation Program, Laboratory visit Good Laboratory Practices
    Regulatory issues in Molecular Diagnostics
    Sample collection, storage and transport Pre-processing of clinical samples for molecular diagnosis, ZN staining
    Introduction to state of art instruments Nucleic acid extraction from clinical samples by silica columns
    Nucleic acid amplification by Real-Time PCR, Software demonstration and analysis of results for different clinical parameters
    Applications of Real-Time PCR Quantitative estimation of pathogen load in clinical samples
    Hybridization based assays for molecular diagnostics Fluorescence In situ Hybridization
    Application of Nucleic acid amplification testing in Disease (Genetic, Infectious, Autoimmune) diagnosis, HLA Typing and Matching
    Biosafety levels and Clinical waste management
    Presentation/ Report submission and Viva voce

    Nano Biotechnology (Specialized) (NB)

    Module Content
    PDA Media Preparation
    Microscopic identification of fungi by Lacto phenol cotton blue (LCB) staining method
    Inoculation of Fungus in PDA Media
    Preparation of cationic liposome bulb.
    Preparation of anionic liposome bulb.
    Preparation of neutral liposome bulb.
    Entrapment of protein in the liposome.
    Entrapment of DNA in the liposome.
    Estimating entrapment efficiency of Protein. Transfer the fungus in to autoclave distilled water
    Estimating entrapment efficiency of DNA.
    Rapid Synthesis of Silver Nanoparticles (from Fungi) :Effect of pH
    Characterization of Silver Nanoparticles by U.V Visible Spectrophotometer

    Plant Biotechnology (Specialized) (PB)

    Module Content
    Introductory Lecture on GLP Demonstration and Handling of Instruments
    Introduction to Plant Biotechnology & Plant Tissue Culture *Formation of groups & allotment of their work
    Sterilization techniques specific to plant culture
    Preparation of culture media for plants
    Preparation of Hormone Stocks Solutions, preparation of PTC hood
    Explant Preparation and Surface Sterilization
    Callus Culture, meristem culture, Micropropagation by Shoot Tip (Explant)
    Preparation of cotton bed & filter paper bridge for in vitro plant culture
    Stress treatment to seeds & viability checking by TTC method
    Seed sterilization, inoculation & stress induction (Drought & Salt stress)
    Morphology study of seedlings grown in vitro
    Enzyme isolation from plant sample & Antioxidant Assay (GPOX) (Control & Stressed plant)
    Preparation of reagents & protein isolation from plant sample (Control & Stressed plant)
    Determination of protein by Bradford method
    Preparation of report & presentation

    Modules/Duration

    Training& Dissertation Timeline

    Program Duration
    Standardized training 2 weeks
    Specialized training 4 weeks
    Project work & Dissertation 1 month
    1.5 month
    2 month
    2.5 month
    3 month
    4-6 month
    1 year

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