Tag

gene editing

Browsing

In terms of World statistics of hunger, food security is one of the biggest problems. The threat of regeneration of availability of food is now increasing day by day. And this is happening not only with small nations but also with developing nations like India. 

The persistence of food insecurity and malnutrition is an on-going issue affecting millions of lives. Even with the success of the green revolution, the world zero hunger motive is not fulfilled. Global hunger, a mortifying crisis is a condition in which people do not get sufficient food to eat. 

They are far away from winning the battle of food security for hundreds to millions of poor people. To combat this problem, Biotechnology provides a tool that enables farmers to grow high yielding crops that even have higher nutritional content, and which can hold out against biological and physical stresses. Biotechnology put forward new solutions to control and overcome these challenges.

Developments in various branches of biotechnology also play an important role in substantially increasing its efforts to find an alternative to synthetic feeding and new innovations in conventional plant breeding technologies. 

Moreover, state-of-the-art biotechnology offers an exciting new, and environmentally friendly way to meet customer demand for sustainable agricultural growth. These sectors of biotechnology play an important role in the fight against hunger in the world:-

Plant Biotechnology

Traditional plant breeding approaches have certain limitations. Since micronutrient malnutrition leads to serious health problems, one intervention is to produce basic food crops for higher levels of micronutrients, also called bio-fortification’. 

These biofortified cultures have been successfully disseminated in a variety of developing countries with proven nutritional and health benefits. In addition, transgenic approaches allow us to reach much higher micronutrient levels in crops than conventional methods alone, thereby increasing nutritional efficiency. 

Genetic engineering can also assist in combining micronutrient traits with agronomic traits that enhance productivity, such as drought tolerance and pest resistance, which are becoming increasingly relevant to climate change.

Nanotechnology 

Nanotechnology impacts many aspects of food science, from the way food, is grown to how it is packaged. Companies are developing nano-material that will make a difference not only in the taste of food but also in food security and the health benefits that food brings. 

The three most promising developments that deploy nanoparticles that enhance the potential of plants to absorb nutrients from the soil are 1. 

  1. Nanocapsules that release a regular supply of pesticides, 
  2. Nano-sensors which measure and adjust soil humidity levels. 
  3. Nanofertilizer which boosts crops with nutrients

In a survey earlier this year, they found that zinc oxide and titanium dioxide nanoparticles increased the levels of antioxidant lycopene in tomatoes to 113 percent. Other researchers explore nanoparticles that protect plants from insects, fungi, and weeds. 

Genetic Engineering

Plants are genetically engineered to modify their DNA sequences to generate certain beneficial traits. Genetic engineering can enhance crop performance and increase the production of the target crop. Crops can even be designed to be more nutritious, providing essential vitamins to people who are struggling to obtain the specific nutrients needed for healthy living. 

GM crops like golden rice, pomato, corn, alfalfa, etc. are some examples of genetically modified crops with high nutritional value. 

Conclusion

The way to eradicate hunger in the world is more complex than any solution and is in fact much more complex than to tackle only the quantity or quality of food. The United Nations Global Sustainable Development Goals address hunger worldwide under Goal 2: Zero Hunger. Their aim is to eradicate hunger, secure food, improve nutrition, and promote sustainable agriculture. This objective lays the foundation for the fight against global hunger through a multi-pronged approach. 

The editing of genes alters a living cell’s genetic material (DNA or RNA). In order to add, delete, or replace individual genetic bases and sequences, it utilizes a variety of different methods and techniques. In medicine, the gene editing process has facilitated the study of diseases in detail, helping clinicians and researchers to understand their root causes. 

The most significant aspect of gene editing is this emphasis on causes as well as on treatment. Although gene editing has been used mainly as a medical biotechnology, it also has exciting applications in many other areas, including agriculture and biofuels, where it can produce more disease-resistant strains of crops or algae. 

As it impacts the building blocks of life, gene editing is a controversial technology, sometimes raising public concerns. However, its growing usage cannot be overlooked and, if potential negative impacts are to be handled, knowledge of its applications is important. Enzymes, particularly nucleases that have been engineered to target a specific DNA sequence, are used to edit genes, where they introduce cuts into the DNA strands, allowing existing DNA to be extracted and replacement DNA added. 

To do this, scientists use various technologies. Such techniques behave like scissors, cutting the DNA at a particular location. Then the DNA where it was cut can be extracted, inserted, or substituted by scientists. In the late 1900s, the first technologies for genome editing were established. More recently, DNA editing has been made simpler than ever by a modern genome editing technique named CRISPR, invented in 2009.

Correcting Genetic Mistakes to Invention of Gene Therapy:

In the genetic discovery period of the mid-20th century, researchers discovered that the sequence of bases in DNA is transmitted from parent to offspring. Recognition of the latter led to the inescapable conjecture that the means to fix those errors would come with the discovery of “molecular errors” that cause genetic diseases and thus allow disease prevention or reversal. 

The underlying concept behind gene therapy was the notion which was used in molecular genetics as a holy grail from the 1980s. However, it has proven difficult to establish gene editing technology for gene therapy. Many early developments focused not on resolving genetic errors in the DNA, but rather on trying to minimize their effect by supplying a functional copy of the mutated gene, either incorporated into the genome or retained as an additional chromosomal unit (outside the genome).

Process:

Genome editing is a procedure where the genetic code of an organism is modified. Researchers use enzymes to ‘cut’ DNA to create a double-strand break (DSB). Non-homologous end joining (NHEJ) or homology-directed repair occurs via DSB repair (HDR). NHEJ creates random gene knockout mutations, while HDR uses extra DNA to construct a desired sequence within the genome (gene knock-in). 

There are four Gene Editing Techniques: Tools to Change the Genome:

Sr.No. Techniques Principle
1 Restriction Enzymes: the native Gene editor  In the 1970s, with the discovery of restriction enzymes, the ability to edit genes became a reality. Restriction enzymes identify and cut unique nucleotide sequence patterns at that site, providing a chance to inject new DNA material at that location.
2 Zinc Finger Nucleases (ZFNs): Increasing  identification ZFNs consist of two parts: an engineered nuclease (Fokl) fused to the DNA-binding domains of the zinc finger. A 3-base pair site on DNA is identified by the zinc-finger DNA-binding domain and can be merged to identify longer sequences.
3 TALENs Gene Editing:

Potentiality within single nucleotide

Transcription activator-like nucleases of effectors (TALENs) are similar to ZFNs structurally. Both methods use the Fokl nuclease to cut DNA and involve functioning dimerization, but the DNA binding domains vary. TALENs, tandem arrays of 33-35 amino acid repeats, use transcription activator-like effectors (TALEs).
4 CRISPR-Cas9 Gene Editing: The game changer CRISPR consists of a guide RNA and a Cas9 nuclease and is a two-component system. Within the ~20 nucleotide region identified by the guide RNA, the Cas9 nuclease cuts the DNA. Knocking out particular genes in cell lines to interrogate gene activity is one of CRISPR’s most commonly used applications.

 

Promoting the Sustainable Development Goals (SDGs):

Gene editing has the ability for many of the SDGs to be advanced. Some examples of areas of application across a wide range of sectors are given below. 

SD Goals Applications
2. Zero hunger Develop the ability of crops to thrive in areas constrained by capital. 

Manage in a humane and ethical way the stock and productivity of livestock.

3. Good health and wellbeing Instead of treating symptoms, which is the current emphasis of most medical medicine, cure or stop diseases. Within the larger trend towards providing genomic medicine, studying the genetic make-up of a person will determine whether a patient will respond well to a drug treatment and allow targeted treatments that reduce unpleasant or harmful side effects.  
6. Clean Water and Sanitation Dissimal and removal by gene editing tools and systems biology of the persistent xenobiotic portion from water have emerged as the outstanding alternative. To overcome the difficulties in the field of bio-remediation of recalcitrant substances from the environment, several bioremediation approaches are present.
7. Affordable and clean energy Develop new sources of energy by allowing organisms to generate biofuels more effectively, such as bio ethanol. This would help minimize reliance on energy sources that are not renewable or detrimental to the environment, such as fossil fuels.
13. Climate action To enhance the use of photosynthesis, gene editing plants may become even more effective in trapping and sequestering carbon from the air.

 

Conclusion:

Advances in genome editing methods have opened new doors to what genome editing can do to solve medicine, agriculture, and beyond problems. CRISPR has fully revolutionized what genome editing, by growing the pace and scope of research, will mean for our future. In its role in drug development, diagnostics, and gene drives, we are already feeling the impact of CRISPR, just to name a few. At this pace, don’t be shocked if in the near future you see more discussion about genome editing.