Bioservice - A Platform for Contract Research

Microbiology

• Isolation & Identification of microorganisms (bacteria, fungus) from various source samples.
• Microscopic, biochemical & serological identification upto species level via atomization by vitek-2
• Drug susceptibility testing (Resistogram typing) of microbes, MIC determination via manual and automation.
• Microbial growth kinetics study- optimization of growth kinetics study – optimization of growth conditions – i.e. pH, temperature, salinity and other stress conditions.
• Typing of bacteria based on their resistogram i.e. Drug resistant, multiple drug resistance (MDR, XDR, MRSA, ESBL, URE)
• Typing of bacteria based on their various medicinal or industrial potential
• Potential as health hazard, diagnostic parameters, their antimicrobial – bactericidal, fungicidal potential, probiotic potential.
• Microbiology contamination determination in any sample like coliform analysis in various water sources, microbial load, colony forming units. CFU determination etc.
• Antimicrobial activity/ potential of any compound – pure or crude like – herbal compounds, chemical compounds, nanoparticles or any other physical or chemical exposure.
• Microscopic examination of microbes or their event via light microscopy & fluorescent microscopy.
• Fluorescent microscopy of fixed slides.
• Screening of microbes for their various potential like industrial health, pollution control, bioremediation, dye degradation, nanoparticle synthesis etc.
• Hospital/ O.T. sterilization Quality control.
• Air contamination by pathogen.
• Microbial quality control/ services by rubbing method – like Hospitals, Operation theatres, instruments etc.
• Drug diffusion assays
• Revival & purification of significant bacterial & fungal microorganisms.
• Antituberculosis activity of various potential future drug compounds.
• Preparation of crude or specific antigen extracts of pathogenic bacteria for various biotechnology experiment/ evaluation.

Biochemistry

Area covered –

• Analysis of various biological fluids for different metabolites including plasma/serum glucose, serum cholesterol, serum protein, serum urea nitrogen, CSF and urinary protein, serum cratinine etc
• Herbal extract preparation
• Antioxidant/free radical analysis of potential compound
• Analysis of secondary metabolites – phytochemical structure of compound
• Quantitation of various cellular components such as protein, carbohydrates, lipids, nucleic acids, vitamins, enzyme etc
• Chromatographic characterization

List of techniques –

• Determination of plasma/serum glucose.
• Determination of total serum protein.
• Determination of CSF and urinary protein.
• Determination of serum cholesterol.
• Determination of serum urea nitrogen
• Determination of serum Creatinine.
• Preparation of plant material for Herbal extract preparation
• Extraction techniques to prepare Herbal extract from Medicinal
• Qualitative phytochemical screening
• Superoxide anion radical scavenging (SO) assay
• Hydroxyl radical scavenging (HO) assay
• Hydrogen peroxide radical scavenging assay
• Reducing power ability
• Non enzymatic antioxidant assay including SOD, CAT etc
• Determination of Total lipid content in serum sample.
• Determination of Pyruvate content in given plant sample.
• Determination of Total soluble sugar content in given plant sample.
• Determination of Total protein content in given plant sample.
• Determination of Vitamin C content in given plant sample.
• Determination of Vitamin E content in given plant sample.
• Determination of phenolic content in given plant sample.
• Determination of flavanoid content in given plant sample.
• DPPH free radical scavenging assay
• Chelating activity assay
• Thin layer chromatography (TLC) for identify un known compound

Molecular Biology

• Isolation & characterization of nucleic acid extracts like DNA, RNA & Plasmids.
• Qualitative & Quantitative analysis of nucleic acid extracts – DNA, RNA, & plasmids
• Genotyping experiments – like mutation detection, PCR, Nested PCR, RT-PCR allele specific amplification, RAPD
• RFLP analysis
• Genomic & Proteomic analysis
• Genotoxicity analysis- DNA cleavage & protein denaturation analysis
• Protein isolation, characterization, Qualitative & quantitative analysis/ profiling
• Molecular identification of micro-organism 16S/18S RNA sequencing
• Gene expression – transcription analysis
• Protein expression analysis
• Gel electrophoresis experiments – agarose, SDS PAGE, NATIVE PAGE etc.
• Primer efficiency test to evaluate U- optimize the primer for PCR & RT-PCR
• Cytokine expression analysis
• ELISA based experiments
• Genetic characterization of bioremediation dye degradation, oil degradation, pesticide degradation any other significant potential of microbes.

Plant Biotechnology/ Plant Tissue Culture

• Phytochemical analysis of various plant extracts such as
  • Antioxidants.
  • Phenolics
  • Flavanoids.
  • Free radical scavenging assay
  • Superoxide
  • Dismuters activity (SOD)
  • Nitric oxide activity, et
• Analysis of chemical compounds on plant growth
• Plant DNA isolation, qualitiative & quantitative estimation
• Plant RNA isolation, qualitative & quantitative estimation
• Molecular Marker Analysis
• Plant Protein Profiling (Isolation and SDS PAGE Run)
• Stress Physiology study of abiotic stresses plants
• Micropropagation
• Qualitative and quantitative analysis of Plant Secondary Metabolite
• Seed Biology
• Plant growth regulatory studies.
• Plant tissue culture based studies.
• Chromatographic separation of potential content

Environment Biotechnology

Water, waste water and soil quality analysis of various physico- chemical & microbiological parameters:

• pH
• Temperature
• Moisture content
• Electrical conductivity
• Salinity
• Dissolved oxygen

Total Solids: Total suspended solids, total dissolved solids, volatile Solids

• BOD
• COD
• NH4+-N
• TKN
• Nitrates
• Nitrites
• Total phosphates
• Total sulphates
• Audity
• Alkalinity
• Hardness
• Percentage of available chlorine in bleaching powder
• Residual chlorine
• Chlorides

Typing of bacteria based on their various industrial potential such as bioremediation of oil, microbiological analysis:

• Total coliforms
• Total Bacteria
• Fecal coliforms
• Pathogens analysis
• Fecal streptococci
• Total bacteria
• Count
• Estimation of moby
• SPC technique

Screening & isolation of microorganisms from the sample for their potential or health hazard