Isolation & Identification of microorganisms (bacteria, fungus) from various source samples.
Microscopic, biochemical & serological identification upto species level via atomization by vitek-2
Drug susceptibility testing (Resistogram typing) of microbes, MIC determination via manual and automation.
Microbial growth kinetics study- optimization of growth kinetics study – optimization of growth conditions – i.e. pH, temperature, salinity and other stress conditions.
Typing of bacteria based on their resistogram i.e. Drug resistant, multiple drug resistance (MDR, XDR, MRSA, ESBL, URE)
Typing of bacteria based on their various medicinal or industrial potential
Potential as health hazard, diagnostic parameters, their antimicrobial – bactericidal, fungicidal potential, probiotic potential.
Microbiology contamination determination in any sample like coliform analysis in various water sources, microbial load, colony forming units. CFU determination etc.
Antimicrobial activity/ potential of any compound – pure or crude like – herbal compounds, chemical compounds, nanoparticles or any other physical or chemical exposure.
Microscopic examination of microbes or their event via light microscopy & fluorescent microscopy.
Fluorescent microscopy of fixed slides.
Screening of microbes for their various potential like industrial health, pollution control, bioremediation, dye degradation, nanoparticle synthesis etc.
Hospital/ O.T. sterilization Quality control.
Air contamination by pathogen.
Microbial quality control/ services by rubbing method – like Hospitals, Operation theatres, instruments etc.
Drug diffusion assays
Revival & purification of significant bacterial & fungal microorganisms.
Antituberculosis activity of various potential future drug compounds.
Preparation of crude or specific antigen extracts of pathogenic bacteria for various biotechnology experiment/ evaluation.
Biochemistry
Area covered –
Analysis of various biological fluids for different metabolites including plasma/serum glucose, serum cholesterol, serum protein, serum urea nitrogen, CSF and urinary protein, serum cratinine etc
Herbal extract preparation
Antioxidant/free radical analysis of potential compound
Analysis of secondary metabolites – phytochemical structure of compound
Quantitation of various cellular components such as protein, carbohydrates, lipids, nucleic acids, vitamins, enzyme etc
Chromatographic characterization
List of techniques –
Determination of plasma/serum glucose.
Determination of total serum protein.
Determination of CSF and urinary protein.
Determination of serum cholesterol.
Determination of serum urea nitrogen
Determination of serum Creatinine.
Preparation of plant material for Herbal extract preparation
Extraction techniques to prepare Herbal extract from Medicinal
Qualitative phytochemical screening
Superoxide anion radical scavenging (SO) assay
Hydroxyl radical scavenging (HO) assay
Hydrogen peroxide radical scavenging assay
Reducing power ability
Non enzymatic antioxidant assay including SOD, CAT etc
Determination of Total lipid content in serum sample.
Determination of Pyruvate content in given plant sample.
Determination of Total soluble sugar content in given plant sample.
Determination of Total protein content in given plant sample.
Determination of Vitamin C content in given plant sample.
Determination of Vitamin E content in given plant sample.
Determination of phenolic content in given plant sample.
Determination of flavanoid content in given plant sample.
DPPH free radical scavenging assay
Chelating activity assay
Thin layer chromatography (TLC) for identify un known compound
Molecular Biology
Isolation & characterization of nucleic acid extracts like DNA, RNA & Plasmids.
Microbiology
Biochemistry
Molecular Biology
Plant Biotechnology/ Plant Tissue Culture
Environment Biotechnology
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