Research Consultant

Bioservice - A Platform for Contract Research

imgMicrobiology

  • Isolation & Identification of microorganisms (bacteria, fungus) from various source samples.
  • Microscopic, biochemical & serological identification upto species level via atomization by vitek-2
  • Drug susceptibility testing (Resistogram typing) of microbes, MIC determination via manual and automation.
  • Microbial growth kinetics study- optimization of growth kinetics study – optimization of growth conditions – i.e. pH, temperature, salinity and other stress conditions.
  • Typing of bacteria based on their resistogram i.e. Drug resistant, multiple drug resistance (MDR, XDR, MRSA, ESBL, URE)
  • Typing of bacteria based on their various medicinal or industrial potential
  • Potential as health hazard, diagnostic parameters, their antimicrobial – bactericidal, fungicidal potential, probiotic potential.
  • Microbiology contamination determination in any sample like coliform analysis in various water sources, microbial load, colony forming units. CFU determination etc.
  • Antimicrobial activity/ potential of any compound – pure or crude like – herbal compounds, chemical compounds, nanoparticles or any other physical or chemical exposure.
  • Microscopic examination of microbes or their event via light microscopy & fluorescent microscopy.
  • Fluorescent microscopy of fixed slides.
  • Screening of microbes for their various potential like industrial health, pollution control, bioremediation, dye degradation, nanoparticle synthesis etc.
  • Hospital/ O.T. sterilization Quality control.
  • Air contamination by pathogen.
  • Microbial quality control/ services by rubbing method – like Hospitals, Operation theatres, instruments etc.
  • Drug diffusion assays
  • Revival & purification of significant bacterial & fungal microorganisms.
  • Antituberculosis activity of various potential future drug compounds.
  • Preparation of crude or specific antigen extracts of pathogenic bacteria for various biotechnology experiment/ evaluation.

imgBiochemistry

Area covered –
  • Analysis of various biological fluids for different metabolites including plasma/serum glucose, serum cholesterol, serum protein, serum urea nitrogen, CSF and urinary protein, serum cratinine etc
  • Herbal extract preparation
  • Antioxidant/free radical analysis of potential compound
  • Analysis of secondary metabolites – phytochemical structure of compound
  • Quantitation of various cellular components such as protein, carbohydrates, lipids, nucleic acids, vitamins, enzyme etc
  • Chromatographic characterization
List of techniques –
  • Determination of plasma/serum glucose.
  • Determination of total serum protein.
  • Determination of CSF and urinary protein.
  • Determination of serum cholesterol.
  • Determination of serum urea nitrogen
  • Determination of serum Creatinine.
  • Preparation of plant material for Herbal extract preparation
  • Extraction techniques to prepare Herbal extract from Medicinal
  • Qualitative phytochemical screening
  • Superoxide anion radical scavenging (SO) assay
  • Hydroxyl radical scavenging (HO) assay
  • Hydrogen peroxide radical scavenging assay
  • Reducing power ability
  • Non enzymatic antioxidant assay including SOD, CAT etc
  • Determination of Total lipid content in serum sample.
  • Determination of Pyruvate content in given plant sample.
  • Determination of Total soluble sugar content in given plant sample.
  • Determination of Total protein content in given plant sample.
  • Determination of Vitamin C content in given plant sample.
  • Determination of Vitamin E content in given plant sample.
  • Determination of phenolic content in given plant sample.
  • Determination of flavanoid content in given plant sample.
  • DPPH free radical scavenging assay
  • Chelating activity assay
  • Thin layer chromatography (TLC) for identify un known compound

img Molecular Biology

  • Isolation & characterization of nucleic acid extracts like DNA, RNA & Plasmids.
  • Qualitative & Quantitative analysis of nucleic acid extracts – DNA, RNA, & plasmids
  • Genotyping experiments – like mutation detection, PCR, Nested PCR, RT-PCR allele specific amplification, RAPD
  • RFLP analysis
  • Genomic & Proteomic analysis
  • Genotoxicity analysis- DNA cleavage & protein denaturation analysis
  • Protein isolation, characterization, Qualitative & quantitative analysis/ profiling
  • Molecular identification of micro-organism 16S/18S RNA sequencing
  • Gene expression – transcription analysis
  • Protein expression analysis
  • Gel electrophoresis experiments – agarose, SDS PAGE, NATIVE PAGE etc.
  • Primer efficiency test to evaluate U- optimize the primer for PCR & RT-PCR
  • Cytokine expression analysis
  • ELISA based experiments
  • Genetic characterization of bioremediation dye degradation, oil degradation, pesticide degradation any other significant potential of microbes.

img Plant Biotechnology/ Plant Tissue Culture

  • Phytochemical analysis of various plant extracts such as
    • Antioxidants.
    • Phenolics
    • Flavanoids.
    • Free radical scavenging assay
    • Superoxide
    • Dismuters activity (SOD)
    • Nitric oxide activity, et
  • Analysis of chemical compounds on plant growth
  • Plant DNA isolation, qualitiative & quantitative estimation
  • Plant RNA isolation, qualitative & quantitative estimation
  • Molecular Marker Analysis
  • Plant Protein Profiling (Isolation and SDS PAGE Run)
  • Stress Physiology study of abiotic stresses plants
  • Micropropagation
  • Qualitative and quantitative analysis of Plant Secondary Metabolite
  • Seed Biology
  • Plant growth regulatory studies.
  • Plant tissue culture based studies.
  • Chromatographic separation of potential content

imgEnvironment Biotechnology

Water, waste water and soil quality analysis of various physico- chemical & microbiological parameters:
  • pH
  • Temperature
  • Moisture content
  • Electrical conductivity
  • Salinity
  • Dissolved oxygen
Total Solids: Total suspended solids, total dissolved solids, volatile Solids
  • BOD
  • COD
  • NH4+-N
  • TKN
  • Nitrates
  • Nitrites
  • Total phosphates
  • Total sulphates
  • Audity
  • Alkalinity
  • Hardness
  • Percentage of available chlorine in bleaching powder
  • Residual chlorine
  • Chlorides
Typing of bacteria based on their various industrial potential such as bioremediation of oil, microbiological analysis:
  • Total coliforms
  • Total Bacteria
  • Fecal coliforms
  • Pathogens analysis
  • Fecal streptococci
  • Total bacteria
  • Count
  • Estimation of moby
  • SPC technique
Screening & isolation of microorganisms from the sample for their potential or health hazard
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