Bioservices

Bioservice - A Platform for Contract Research

• Microbiology

  • Isolation & Identification of microorganisms (bacteria, fungus) from various source samples.

  • Microscopic, biochemical & serological identification upto species level via atomization by vitek-2

  • Drug susceptibility testing (Resistogram typing) of microbes, MIC determination via manual and automation.

  • Microbial growth kinetics study- optimization of growth kinetics study – optimization of growth conditions – i.e. pH, temperature, salinity and other stress conditions.

  • Typing of bacteria based on their resistogram i.e. Drug resistant, multiple drug resistance (MDR, XDR, MRSA, ESBL, URE)

  • Typing of bacteria based on their various medicinal or industrial potential

  • Potential as health hazard, diagnostic parameters, their antimicrobial – bactericidal, fungicidal potential, probiotic potential.

  • Microbiology contamination determination in any sample like coliform analysis in various water sources, microbial load, colony forming units. CFU determination etc.

  • Antimicrobial activity/ potential of any compound – pure or crude like – herbal compounds, chemical compounds, nanoparticles or any other physical or chemical exposure.

  • Microscopic examination of microbes or their event via light microscopy & fluorescent microscopy.

  • Fluorescent microscopy of fixed slides.

  • Screening of microbes for their various potential like industrial health, pollution control, bioremediation, dye degradation, nanoparticle synthesis etc.

  • Hospital/ O.T. sterilization Quality control.

  • Air contamination by pathogen.

  • Microbial quality control/ services by rubbing method – like Hospitals, Operation theatres, instruments etc.

  • Drug diffusion assays

  • Revival & purification of significant bacterial & fungal microorganisms.

  • Antituberculosis activity of various potential future drug compounds.

  • Preparation of crude or specific antigen extracts of pathogenic bacteria for various biotechnology experiment/ evaluation.

• Molecular Biology

  • Isolation & characterization of nucleic acid extracts like DNA, RNA & Plasmids.

  • Qualitative & Quantitative analysis of nucleic acid extracts – DNA, RNA, & plasmids

  • Genotyping experiments – like mutation detection, PCR, Nested PCR, RT-PCR allele specific amplification, RAPD

  • RFLP analysis

  • Genomic & Proteomic analysis

  • Genotoxicity analysis- DNA cleavage & protein denaturation analysis

  • Protein isolation, characterization, Qualitative & quantitative analysis/ profiling

  • Molecular identification of micro-organism 16S/18S RNA sequencing

  • Gene expression – transcription analysis

  • Protein expression analysis

  • Gel electrophoresis experiments – agarose, SDS PAGE, NATIVE PAGE etc.

  • Primer efficiency test to evaluate U- optimize the primer for PCR & RT-PCR

  • Cytokine expression analysis

  • ELISA based experiments

  • Genetic characterization of bioremediation dye degradation, oil degradation, pesticide degradation any other significant potential of microbes.

• Plant Biotechnology/ Plant Tissue Culture

  • Phytochemical analysis of various plant extracts such as

    Antioxidants.

    Phenolics

    Flavanoids.

    Free radical scavenging assay

    Superoxide

    Dismuters activity (SOD)

    Nitric oxide activity, etc.

  • Analysis of chemical compounds on plant growth

  • Plant DNA isolation, qualitiative & quantitative estimation

  • Plant RNA isolation, qualitative & quantitative estimation

  • Molecular Marker Analysis

  • Plant Protein Profiling (Isolation and SDS PAGE Run)

  • Stress Physiology study of abiotic stresses plants

  • Micropropagation

  • Qualitative and quantitative analysis of Plant Secondary Metabolite

  • Seed Biology

  • Plant growth regulatory studies.

  • Plant tissue culture based studies.

  • Chromatographic separation of potential content

• Environment Biotechnology

  • Water, waste water and soil quality analysis of various physico- chemical & microbiological parameters:

    pH

    Temperature

    Moisture content

    Electrical conductivity

    Salinity

    Dissolved oxygen

  • Total Solids: Total suspended solids, total dissolved solids, volatile Solids

    BOD

    COD

    NH4+-N

    TKN

    Nitrates

    Nitrites

    Total phosphates

    Total sulphates

    Audity

    Alkalinity

    Hardness

    Percentage of available chlorine in bleaching powder

    Residual chlorine

    Chlorides

  • Typing of bacteria based on their various industrial potential such as bioremediation of oil, microbiological analysis:

    Total coliforms

    Total Bacteria

    Fecal coliforms

    Pathogens analysis

    Fecal streptococci

    Total bacteria

    Count

    Estimation of moby

    SPC technique

  • Screening & isolation of microorganisms from the sample for their potential or health hazard

• Biochemistry

  • Area covered –

    Analysis of various biological fluids for different metabolites including plasma/serum glucose, serum cholesterol, serum protein, serum urea nitrogen, CSF and urinary protein, serum cratinine etc

    Herbal extract preparation

    Antioxidant/free radical analysis of potential compound

    Analysis of secondary metabolites – phytochemical structure of compound

    Quantitation of various cellular components such as protein, carbohydrates, lipids, nucleic acids, vitamins, enzyme etc

    Chromatographic characterization

  • List of techniques –

    Determination of plasma/serum glucose.

    Determination of total serum protein.

    Determination of CSF and urinary protein.

    Determination of serum cholesterol.

    Determination of serum urea nitrogen

    Determination of serum Creatinine.

    Preparation of plant material for Herbal extract preparation

    Extraction techniques to prepare Herbal extract from Medicinal

    Qualitative phytochemical screening

    Superoxide anion radical scavenging (SO) assay

    Hydroxyl radical scavenging (HO) assay

    Hydrogen peroxide radical scavenging assay

    Reducing power ability

    Non enzymatic antioxidant assay including SOD, CAT etc

    Determination of Total lipid content in serum sample.

    Determination of Pyruvate content in given plant sample.

    Determination of Total soluble sugar content in given plant sample.

    Determination of Total protein content in given plant sample.

    Determination of Vitamin C content in given plant sample.

    Determination of Vitamin E content in given plant sample.

    Determination of phenolic content in given plant sample.

    Determination of flavanoid content in given plant sample.

    DPPH free radical scavenging assay

    Chelating activity assay

    Thin layer chromatography (TLC) for identify un known compound